P-63: The Effect of Temperatures on Goat Epididymal Sperm Motility in Tissue Culture Media 199

Background: There is a general deterioration in sperm quality depending on time postmortem. Refrigeration of the epididymes down to around 5°C is the best strategy to lower this damage and there are many dissim ilarities between species, possibly due to differences on cold shock endurance of epididymal sperm. Our objective was to determine the effect of 5°C and 37°C on goat epididymal sperms motility during 24 hours in TCM199 Materials and Methods: Sperm were collected performing several incisions in the cauda epididymis of goat testicles, and taking the white fluid emerging into micro tube containing TCM 199 with 10% bovine serum albumin. After count (30×106 sperm/ml), one aliquots kept at 5°C refrigerator and the other incubated at 37°C. Sperm motility was examined at 1, 6, 12, and 24 hours after being kept at 5°C refrigerator and 37°C incubator with computer-assisted sperm analyzers (CASA). Statistical analysis were carried out using the one-way ANOVA of SPSS version 21. Results: Till 6 hours CASA parameters; motile percentage (Class A+B+C, %), straight line velocity (VSL, μm/s), curvilinear velocity (VCL, μm/s), average path velocity (VAP, μm/s), amplitude of lateral head displacement (ALH, μm), angular displacement (MAD, D), were significantly higher in 37°C group than 5°C group (p<0.05). But, at 6-12 and 24 hours., rapid progressive motility (Class A, %), progressive motility (class A+B), motile percentage,VSL,VCl,VAP,ALH, and BCF were significantly higher in 5°C group than 37°C group (p<0.05). Conclusion: Motility parameter of goat epididymal sperm can be kept at 37°C in TCM 199 only till 6 hours. But when preserved at 5°C in TCM 199, it can be kept till 24 hours.